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To investigate the differences in intestinal microbiota of diarrheic and healthy sika deer fawns nursed artificially, and to identify potential pathogenic bacterial taxa associated with diarrhea, fecal samples from six diarrheic and six healthy fawns were collected and analyzed using 16S rRNA gene sequencing. The results revealed that the intestinal microbial diversity in the diarrheic group was significantly lower than that in the healthy group. In the diarrheic fawns, the relative abundances of dominant phyla such as Firmicutes and Bacteroidetes were reduced, whereas those of opportunistic pathogens belonging to Fusobacteria and Proteobacteria were elevated. At the genus level, increased abundances of Enterobacterium, Fusobacterium, and Klebsiella were observed. These findings suggest that the primary cause of diarrhea in artificially reared sika deer fawns is dysbiosis characterized by a decrease in beneficial commensal bacteria and an overrepresentation of opportunistic pathogenic genera, including Enterobacterium and Fusobacterium.
[1]孙红瑜.仔鹿下痢的病因分析及综合防治[J].现代畜牧科技,2020(4):129-130.
[2]张玉莹,刘书杰,冯宇哲,等.基于16S rRNA测序分析正常与腹泻牦牛犊牛粪便细菌区系组成[J].中国畜牧兽医,2021,48(9):3293-3302.
[3]邵清,邴新帅,刘红洋,等.哺乳仔鹿大肠杆菌性下痢的防治体会[J].当代畜牧,2024(2):126-127.
[4]SCIUMèG D, BERTI G, LAMBIASE C, et al. Misinterpreting diarrhea-predominant irritable bowel syndrome and functional diarrhea:pathophysiological highlights[J]. Journal of Clinical Medicine, 2023, 12(18):5787.
[5]张子敬,朱肖亭,吕世杰,等.腹泻犊牛与健康犊牛粪便菌群结构组成与功能研究[J].中国畜牧兽医,2020, 47(9):2779-2788.
[6]胡世超.腹泻湖羊羔羊粪便菌群多样性研究[D].张家口:河北北方学院,2023.
[7]李常营,徐兰梦,黄榆智,等.哺乳方式对猪生长、血清生化、肠道微生物及代谢物的影响[J].畜牧兽医学报,2024, 55(11):5147-5158.
[8]王琦,陈巧利,肖图雄,等. 1例梅花鹿感染大肠埃希菌、弗格森埃希菌及肠杆菌属细菌死亡的病例报告[J].贵州畜牧兽医,2024, 48(5):73-75.
[9]元虹懿.东北梅花鹿夏季和冬季肠道菌群变化及其功能差异分析[D].哈尔滨:东北林业大学,2022.
[10]吴家慧,石明慧,王一晨,等.冬末春初圈养梅花鹿健康与腹泻个体粪便菌群的比较分析[J].微生物学杂志,2023, 43(2):68-77.
[11]李鹏飞. 0~56日龄成都麻羊羔羊肠道微生物的定植与变化规律[D].雅安:四川农业大学,2020.
[12]TANG C, LIU Z J, LU L, et al. The effects of formula milk on development, nutrient absorption, and immune characteristics of piglets[J]. BMC Genomics, 2025, 26(1):732.
[13]徐怡亮.腹泻仔猪肠道微生物结构及粪便代谢组学研究[D].合肥:安徽农业大学,2020.
[14]J?RVINEN-SEPPO K M. Effect of feeding human milk on development of the infant immune system and allergic outcomesan area of research challenge and need[J]. Breastfeeding Medicine, 2025, 20(4):201-204.
[15]PANNARAJ P S, LI F, CERINI C, et al. Association between breast milk bacterial communities and establishment and development of the infant gut microbiome[J]. JAMA Pediatrics,2017, 171(7):647.
[16]ZHU Y Q, CHEN B R, ZHANG X Y, et al. Exploration of the Muribaculaceae family in the gut microbiota:diversity,metabolism, and function[J]. Nutrients, 2024, 16(16):2660.
[17]董宁.腹泻羔羊的肠道微生物检测分析及抗生素治疗的影响研究[D].杨凌:西北农林科技大学,2022.
[18]VARADA V V, KUMAR S, CHHOTARAY S, et al. Hostspecific probiotics feeding influence growth, gut microbiota, and fecal biomarkers in buffalo calves[J]. AMB Express, 2022, 12(1):118.
[19]刘波.多组学联合研究复合益生菌对新生犊牛肠道菌群多样性及机体代谢的作用机制[D].呼和浩特:内蒙古农业大学,2023.
[20]?GREN E, BACKHANS A, LINDBERG M, et al. Antimicrobial resistance in Escherichia coli from Swedish piglets with diarrhoea and associations with potential risk factors[J]. Acta Veterinaria Scandinavica, 2025, 67(1):16.
[21]赵清梅,崔省委,郭仕辉,等.腹泻犊牛粪便菌群及粪便代谢物的变化特征分析[J].中国畜牧兽医,2024, 51(9):4092-4105.
[22]崔一喆,王秋菊,苏景,等.复合益生菌制剂对离乳梅花鹿生长性能、粪便菌群及血液生化指标的影响[J].中国兽医学报,2017, 37(1):129-133, 143.
[23]刘春艳.猪源罗伊氏乳杆菌对断奶仔猪肠道屏障功能和细胞外基质的影响[D].南宁:广西大学,2023.
[24]刘英玉,郑晓琴,李瑞鹏,等.新疆某奶牛场犊牛腹泻病原菌检测及鉴定[J].家畜生态学报,2023, 44(11):68-71, 86.
[25]孙绘程.仔鹿腹泻源沙门氏菌、奇异变形杆菌分离鉴定及耐药分析[D].长春:吉林农业大学,2023.
[26]杨春蕾.吉林省部分地区三种仔鹿腹泻病原菌分离鉴定及耐药性分析[D].长春:吉林农业大学,2023.
[27]赵欣雅,田雨佳,陈星星,等.普雷沃氏菌在瘤胃内的作用与代谢机制研究进展[J].饲料研究,2023, 46(14):151-153.
[28]张洁,张力莉,徐晓锋,等.反刍动物瘤胃内普雷沃氏菌的研究进展[J].中国饲料,2020(7):17-21.
[29]杜欣颖,夏介英,李东晓,等.健康与腹泻林麝肠道菌群的多态性[J].中国微生态学杂志,2021, 33(7):745-751.
[30]WANG W Y, WANG Q L, CAO R L, et al. Metagenomic investigation of pathogenic RNA viruses causing diarrhea in sika deer fawns[J]. Viruses, 2025, 17(6):803.
Basic Information:
DOI:10.19848/j.cnki.ISSN1005-2739.2025.12.0006
China Classification Code:S858.25
Citation Information:
[1]ZHANG Wang,GUO Yating,GUO Xingjian ,et al.Analysis of the differences in intestinal flora of diarrhea and healthy sika deer nursed artificially[J].Heilongjiang Journal of Animal Reproduction,2025,33(06):35-43.DOI:10.19848/j.cnki.ISSN1005-2739.2025.12.0006.
Fund Information:
国家重点研发计划项目(2023YFD1302001)
2025-12-15
2025-12-15