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The aim of this experiment was to analyze the effects of in vitro culture methods, pituitary FSH(pFSH) pretreatment, and physiological status on Holstein bovine OPU-IVEP efficiency. In the experiment, two different in vitro culture systems(single-step and two-step culture methods), pFSH from different sources of(N-pFSH, S-pFSH, and B-pFSH), and different physiological conditions(non-pregnant and early pregnant) were chosen to evaluate the developmental competence of OPUderived oocytes in Holstein bovine. The results showed that compared with the singlestep culture method, the two-step culture method could significantly increase the cleavage rate[(82. 7±3. 8)% vs.(75. 6±5. 7)%,P<0. 05] and A-grade blastocyst rate[(37. 2±2. 7)% vs.(27. 8±2. 9)%, P<0. 05], and no significant difference in the blastocyst rate between the two culture methods [(55. 1±2. 6)% vs.(49. 2±1. 8)%, P>0. 05]. Compared with S-pFSH and B-pFSH, N-pFSH pre-treatment significantly increased the average number of viable oocytes [(26. 5±2. 9)% vs.(17. 3±2. 2)% vs.(15. 8±2. 6)%, P<0. 05], and blastocyst rates and A-grade blastocyst rate were similar among all groups(P>0. 05). In addition, the similar average number of viable oocytes, cleavage rate, blastocyst rate and A-grade blastocyst rate in early-pregnancy donor was observed, compared to non-pregnancy donor(P>0. 05). In summary, N-pFSH pre-treatment combined with the two-step culture method could improve the OPU-IVEP efficiency, and early-pregnancy cows can also be effectively used for OPU-IVEP.
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Basic Information:
DOI：10.19848/j.cnki.ISSN1005-2739.2026.08.0006
China Classification Code:S823
Citation Information:
[1]Song Yukun,Gao Xinxin,Kang Yin ,et al.Analysis of factors affecting the efficiency of bovine OPU-IVEP[J].Heilongjiang Journal of Animal Reproduction,2026,34(04):28-33.DOI:10.19848/j.cnki.ISSN1005-2739.2026.08.0006.
Fund Information:
内蒙古自治区揭榜挂帅项目（2022JBGS0025）
2026-08-15
2026-08-15