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The experiment aimed to explore the effect of adding empagliflozin(Empa) to the cryopreserved diluent on the insemination of frozenthawed semen from Chengde hornless goats. Ninety healthy multiparous ewes aged 2 to 3 y were randomly divided into control group(n=45) and experimental group(n=45). The frozen semen from control group was thawed using self-prepared cryopreservation dilution solution, while that of experimental group was added with 0. 8 μmol/L Empa to the cryopreservation dilution solution. Laparoscopic insemination was performed on the ewes of the two groups respectively. The results showed that the sperm motility and motion parameters such as linear velocity(VSL), curvilinear velocity(VCL), mean path velocity(VAP), and linear(LIN) were significantly improved(P<0. 05) and the pregnancy rate and lambing rate were slightly higher(P>0. 05) in the experimental group than those in the control group. It suggested that the addition of Empa to the cryopreservation dilution solution for the frozen-thawed semen of Chengde hornless goat could effectively alleviate oxidative stress responses, improve sperm quality and the pregnancy rate of artificial insemination.
[1]Sharafi M, Borghei-Rad S M, Hezavehei M, et al.Cryopreservation of semen in domestic animals:a review of current challenges, applications, and prospective strategies[J]. Animals,2022, 12(23):3271.
[2]杨轩宁，赵萌萌，张鲁.人工授精技术在畜牧生产中的应用历程[J].黑龙江动物繁殖，2023, 31(1):43-49, 60.
[3]Silva S V, Soares A T, Batista A M, et al. In vitro and in vivo evaluation of ram sperm frozen in tris egg-yolk and supplemented with superoxide dismutase and reduced glutathione[J].Reproduction in Domestic Animals, 2011, 46(5):874-881.
[4]Uthman L, Li X L, Baartscheer A, et al. Empagliflozin reduces oxidative stress through inhibition of the novel inflammation/NHE/[Na+]c/ROS-pathway in human endothelial cells[J]. Biomedicine&Pharmacotherapy, 2022, 146:112515.
[5]Dana P, Hayati Rodbari N, Yaghmaei P, et al. Effects of empagliflozin on sexual function, testicular histology and biochemical parameters in young and middle-aged diabetic rats of type2[J]. Iranian Journal of Diabetes and Metabolism, 2021, 21(4):210-218.
[6]金美林，蔡元.绵羊精液冷冻保存技术的研究进展[J].甘肃畜牧兽医，2017, 47(3):61-62.
[7]从光雷，刘青，孙伟，等.抗氧化剂在绵羊精液保存中的研究与应用进展[J].中国畜牧杂志，2020, 56(2):1-7.
[8]Baskaran S, Finelli R, Agarwal A, et al. Reactive oxygen species in male reproduction:a boon or a bane?[J]. Andrologia, 2020,52(3). DOI:10. 1111/and. 13577.
[9]Barati E, Nikzad H, Karimian M. Oxidative stress and male infertility:current knowledge of pathophysiology and role of antioxidant therapy in disease management[J]. Cellular and Molecular Life Sciences, 2020, 77(1):93-113.
[10]Banday M N, Ahmad Lone F, Rasool F, et al. Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation[J]. Cryobiology, 2017, 74:25-30.
[11]De Souza C V, Brandão F Z, Santos J D R, et al. Effect of different concentrations of l-carnitine in extender for semen cryopreservation in sheep[J]. Cryobiology, 2019, 89:104-108.
[12]Mu Y, Luo L B, Huang R, et al. Cardiac-derived CTRP9mediates the protection of empagliflozin against diabetes-induced male subfertility in mice[J]. Clinical Science, 2024, 138(21):1421-1440.
[13]张楠，李进冬，孔敏，等.恩格列净对糖尿病小鼠肾组织氧化应激及Nrf2/ARE通路的影响[J].徐州医科大学学报，2021, 41(10):742-746.
[14]Tang Y J, Wu H, Liu L D, et al. Adding lycopene to the freezing media enhances the quality, antioxidant capacity, and fertilization ability of frozen-thawed Oure-type Tibetan sheep(Ovis aries)sperm[J]. Cryobiology, 2026, 122:105578.
[15]Fan Y L, Lv W B, Li X W, et al. Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway[J]. Frontiers in Veterinary Science, 2025, 12:1613506.
[16]Masoudi R, Shahneh A Z, Towhidi A, et al. Fertility response of artificial insemination methods in sheep with fresh and frozenthawed semen[J]. Cryobiology, 2017, 74:77-80.
Basic Information:
DOI：10.19848/j.cnki.ISSN1005-2739.2026.08.0004
China Classification Code:S827
Citation Information:
[1]Pang Zhaoqi,Li Mengqi,Zhang Jinlong ,et al.Effect of adding empagliflozin(Empa) to the cryopreserved diluent on insemination of frozen-thawed semen from Chengde hornless goats[J].Heilongjiang Journal of Animal Reproduction,2026,34(04):19-22.DOI:10.19848/j.cnki.ISSN1005-2739.2026.08.0004.
Fund Information:
“十四五”国家重点研发计划项目（2021YFD1200104）
2026-08-31
2026
2026-09-23
2026
1
2026-08-15
2026-08-15